Hematopoietic and nitrogen sparing effect of preparations from hog gastric mucosa with high content of blood group substances.

penitoneal injection of phenylhydrazine hydrochloride. The mucin preparation was obtained from hog gastric mucosa by tnichloroacetic acid (leproteinization of the aqueous extract followed by acetone precipitation. It was active as intrinsic factor in humans. Further investigations, however, have shown that the hematopoietic effect was not due to an increase of vitamin B,2 or Fe intestinal absorption.3’4 Therefore, it could not be related to the intrinsic factor content of the preparation. This conclusion agrees with the observations of Rosenhlum et al.5 and Chow et al.C on the inactivity of hog intrinsic factor in the rat, a species that probably needs a specific intrinsic factor.7’8 It may be supposed that the above referred antianemic effect is exerted through a sparing action on nitrogen metabolism. Therefore, in order to identify the component ( or components ) responsible for the reported activities,